Introduction to Image Acquisition for Quantitative Analysis
How do we visualize biological samples? In this talk, Dr. Nico Stuurman provides an overview of the different tools, equipment, and software available to acquire an image of a biological sample using a light microscope, and the considerations one needs to take when using these tools. This lecture will allow scientists to understand the principles behind image acquisition in order to improve and optimize the analysis of their sample.
detectors, detector noise, image display, binary image, grayscale values, illumination correction, metadata, file format