Virus clearance and carryover study on twin-column (continuous chromatography) system
On its pathway to clinical and commercial scale, multicolumn capture chromatography requires development of scalable models for virus validation studies. In this study, retro‐ (X‐MuLV) and parvo‐virus (MVM) clearance in twin‐column continuous capture chromatography (CaptureSMB) was investigated and a surrogate model was developed using standard batch mode chromatography. The results showed that the log reduction values (LRVs) achieved during CaptureSMB were also comparable to the LRVs obtained using standard batch capture chromatography and that virus carryover could be well controlled. Further this presentation also shows assessments of the impact of resin cleaning strategy and the duration of clean‐in‐place solution exposure on virus carryover.
This is a ~20 minute presentation after which live audience Q&A will ensue.
NOTE: this is one in a series of ~15 webinars on twin-column “continuous” chromatography technology by YMC/ChromaCon. View all on www.biolingo.com search term “YMC”.
Leaders in multi-column chromatography, YMC/ChromaCon has developed and patented novel process principles, process control and simulation software, equipment designs and operating software for batch, cyclic and continuous chromatography. For mAbs, recombinant proteins, vaccines, oligos and peptides these process solutions result in significant cost reduction and / or productivity, yield and purity improvements which are scalable to GMP chromatographic solutions.
YMC is a Life Science technology company with offices and factories in North America, Asia, India, and Europe. Founded in 1980, and headquartered in Kyoto, Japan, the over 500 employees are providing best-in-class lab and GMP scale process solutions to the bio/pharmaceutical industry; chromatography columns, packing media. resins, LPLC and HPLC bench and GMP scale systems supplier.